Acanthamoeba keratitis is commonly encountered by contact lens wearers. Contact lens solution plays an important role in the safe use of contact lenses. The most popular products for disinfecting lenses are multipurpose disinfecting solutions (MPDS). However, almost all MPDS retailed in Korea are ineffective in killing Acanthamoeba. The objective of this study was to determine the possibility of using autophagy inhibitor chloroquine as a disinfecting agent to improve the amoebicidal activity of MPDS against Acanthamoeba, especially the cyst. Amoebicidal effects of eight different MPDSs combined with chloroquine (CQ), an autophagy inhibitor, and their cytotoxicities to human corneal epithelium cells were determined. Almost all MPDS showed strong amoebicidal effect on trophozoites after 8 h of exposure. However, they showed inadequate amoebicidal effect on cysts even after 24 h of exposure. MPDSs combined with 100 μM CQ increased their amoebicidal effects on immature cyst by inhibiting formation of mature cysts. Incubation with 100 μM CQ for 30 min did not have cytotoxicity to human corneal epithelial cells.
Keywords: Acanthamoeba; Amoebicidal effects; Autophagy inhibitor; Chloroquine; MPDS.
Copyright © 2018. Published by Elsevier Inc.