Architecture of the RNA polymerase II elongation complex: new insights into Spt4/5 and Elf1

Transcription. 2018;9(5):286-291. doi: 10.1080/21541264.2018.1454817. Epub 2018 May 7.

Abstract

Transcription by RNA polymerase II (Pol II) is accomplished with the aid of numerous accessory factors specific to each transcriptional stage. The structure of the Pol II elongation complex (EC) bound with Spt4/5, Elf1, and TFIIS unveiled the sophisticated basal EC architecture essential for transcription elongation and other transcription-related events.

Keywords: Elf1; RNA polymerase II; Spt4/5; TFIIS; X-ray crystallography; cryo-EM; elongation complex; transcription elongation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Archaea
  • Cell Nucleus / chemistry
  • Cell Nucleus / metabolism
  • Computer Simulation
  • Crystallography, X-Ray
  • Eukaryota
  • Molecular Conformation
  • Multiprotein Complexes / chemistry*
  • Multiprotein Complexes / metabolism
  • RNA Polymerase II / chemistry*
  • RNA Polymerase II / metabolism
  • Transcription Elongation, Genetic*
  • Transcriptional Elongation Factors / chemistry*
  • Transcriptional Elongation Factors / metabolism

Substances

  • Multiprotein Complexes
  • Transcriptional Elongation Factors
  • RNA Polymerase II

Grants and funding

This work was supported by Japan Society for the Promotion of Science KAKENHI [grant number JP15H04344].