Metabolic profiling of four South African herbal teas using high resolution liquid chromatography-mass spectrometry and nuclear magnetic resonance

Abstract

Increased preference to herbal drinks has led to global interest in the use and production of different plant species for the preparation of various drink formulations. Medicinal properties derived from bioactive compounds remain the main driver of choice for herbal teas. This study determined the chemical variation in honeybush, rooibos, special and bush tea, profiled compounds responsible for such differences and compared their peak areas. Nuclear magnetic resonance and high resolution liquid chromatography-mass spectrometry were used to determine compound variation and profiling. Principal component analysis and partial-least square multivariate statistical analysis showed distinct differences (P < 0.05) between the different types of herbal teas. Detected compounds included flavonoids, phenolics, lignans, megastigmane glycoside, most of which possess health benefits. The findings showed that South African herbal teas could play a vital role as health promoting drinks, and that bush tea and special tea are phytochemically comparable with other commercialized herbal teas.

Keywords: Bush tea; Caffeine (PubChem CID: 2519); Chrysosplenetin (PubChem CID: 5281608); Eriojaposide A (PubChem CID: 9892275); Herbal tea; Hesperidin (PubChem CID: 10621); Justicidin B (PubChem CID: 442882); Luteolin (PubChem CID: 5280445); Mangiferin (PubChem CID: 5281647); Metabolomics; Myricitrin (PubChem CID: 5281673); NMR; Orientin (PubChem CID: 5281675); Quercetin 3-O-glucoside (PubChem CID: 5748594); Special tea; UPLC-Q-TOF/MS.