Application of NucleoCounter for the comprehensive assessment of murine cultured neurons during infection with Equine Herpesvirus type 1 (EHV-1)

Abstract

The NucleoCounter NC-3000, a portable high-speed cell counting device based on the principle of fluorescence microscopy, provides the alternative method for standard flow cytometry. The main objective of the study was to apply an efficient technique for the assessment of the primary murine neurons culture infected with either neuropathogenic or non-neuropathogenic strains of Equine Herpesvirus type 1 (EHV-1). Using the NucleoCounter NC-3000 we have observed a decrease in mitochondrial potential and reduction in cells viability but we have not observed changes in the cell cycle of cultured neurons infected with all EHV-1 strains.

Keywords: EHV-1; NucleoCounter NC-3000; apoptosis; cell cycle; neurons; viability.