Quadruplex gold immunochromatogaraphic assay for four families of antibiotic residues in milk

Jinyu Zhou; Wei Nie; Yiqiang Chen; Chunjiang Yang; Lu Gong; Chi Zhang; Qian Chen; Lidong He; Xiaoyu Feng

doi:10.1016/j.foodchem.2018.02.002

Quadruplex gold immunochromatogaraphic assay for four families of antibiotic residues in milk

Food Chem. 2018 Aug 1:256:304-310. doi: 10.1016/j.foodchem.2018.02.002. Epub 2018 Feb 21.

Authors

Jinyu Zhou¹, Wei Nie¹, Yiqiang Chen², Chunjiang Yang³, Lu Gong¹, Chi Zhang¹, Qian Chen⁴, Lidong He⁵, Xiaoyu Feng⁶

Affiliations

¹ Beijing Advanced Innovation Center for Food Nutrition and Human Health, and State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
² Beijing Advanced Innovation Center for Food Nutrition and Human Health, and State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. Electronic address: yqchen@cau.edu.cn.
³ NBGene Co Ltd, add, Building 7, Bodaxing Industry Park, BDA, Beijing 101111, China.
⁴ Department of Cancer Genetics and Epigenetics, City of Hope Cancer Center, CA 91010, USA.
⁵ Department of Chemistry and Biochemistry, Florida State University, FL 32306-4390, USA.
⁶ Beijing Centers for Disease Control and Prevention, Beijing 100112, China.

PMID: 29606452
DOI: 10.1016/j.foodchem.2018.02.002

Abstract

In this study, we developed a quadruplex gold immunochromatogaraphic assay (GICA) for the simultaneous determination of four families of antibiotics including β-lactams, tetracyclines, streptomycin and chloramphenicol in milk. For qualitative analysis, the visual cut-off values were measured to be 2-100 ng/mL, 16-32 ng/mL, 50 ng/mL and 2.4 ng/mL for β-lactams, tetracyclines, streptomycin and chloramphenicol, respectively. For quantitative analysis, the detection ranges were 0.13-1 ng/mL for penicillin G, 0.13-8 ng/mL for tetracycline, 0.78-25 ng/mL for streptomycin, 0.019-1.2 ng/mL for chloramphenicol in milk respectively, with linear correlation coefficients higher than 0.97. The spiked experiment indicated that the mean recoveries ranged from 84.5% to 107.6% with coefficient of variations less than 16.2%, and real sample analysis revealed that the GICA can produce consistent results with instrumental analysis. These results demonstrated that this novel immunoassay is a promising approach for rapidly screening common antibiotic residues in milk.

Keywords: Chloramphenicol; Immunochromatogaraphic assay; Milk; Streptomycin; Tetracyclines; β-Lactams.

MeSH terms

Animals
Anti-Bacterial Agents / analysis*
Chromatography, Affinity / methods*
Drug Residues / analysis*
Food Contamination / analysis
Gold / chemistry*
Limit of Detection
Milk / chemistry*

Substances

Anti-Bacterial Agents
Gold