Lupine allergen detecting capability and cross-reactivity of related legumes by ELISA

Martina Koeberl; Michael F Sharp; Rongkai Tian; Saman Buddhadasa; Dean Clarke; James Roberts

doi:10.1016/j.foodchem.2018.02.043

Lupine allergen detecting capability and cross-reactivity of related legumes by ELISA

Food Chem. 2018 Aug 1:256:105-112. doi: 10.1016/j.foodchem.2018.02.043. Epub 2018 Feb 9.

Authors

Martina Koeberl¹, Michael F Sharp², Rongkai Tian³, Saman Buddhadasa⁴, Dean Clarke⁵, James Roberts⁶

Affiliations

¹ National Measurement Institute, 1/153 Bertie Street, Port Melbourne, VIC, 3207 Melbourne, Australia. Electronic address: martina.koeberl@measurement.gov.au.
² National Measurement Institute, 1/153 Bertie Street, Port Melbourne, VIC, 3207 Melbourne, Australia. Electronic address: msharp@svi.edu.au.
³ National Measurement Institute, 1/153 Bertie Street, Port Melbourne, VIC, 3207 Melbourne, Australia; Chemical and Biomedical Engineering, University of Melbourne, Grattan Street, Parkville, VIC, 3010 Melbourne, Australia.
⁴ National Measurement Institute, 1/153 Bertie Street, Port Melbourne, VIC, 3207 Melbourne, Australia. Electronic address: saman.buddhadasa@measurement.gov.au.
⁵ National Measurement Institute, 1/153 Bertie Street, Port Melbourne, VIC, 3207 Melbourne, Australia. Electronic address: dean.clarke@measurement.gov.au.
⁶ National Measurement Institute, 1/153 Bertie Street, Port Melbourne, VIC, 3207 Melbourne, Australia. Electronic address: james.roberts@measurement.gov.au.

PMID: 29606424
DOI: 10.1016/j.foodchem.2018.02.043

Abstract

Lupine belongs to the genus Lupinus and includes three species commonly consumed by humans. The Lupinus genus is closely related to other legumes, such as peanuts, soya, chickpeas, peas, lentils and beans. However, the consumption of lupine (and related legumes) can cause severe allergenic reactions. Therefore, reliable analytical detection methods are required for the analysis of food samples. In this study three commercially available ELISA test kits were analyzed for the detection capability of three common lupine species, as well as cross-reactivity to related legumes. All three ELISA test kits could detect the lupine species, though with different sensitivities. Cross-reactivity varied for the ELISA test kits and all showed some cross-reactivity to related legume samples analyzed.

Keywords: ELISA test kit comparison; Lupine cross-reactivity; Lupinus albus (White Lupine); Lupinus angustifolius (Blue Lupine or Australian Sweet Lupine); Lupinus luteus (Yellow Lupine).

MeSH terms

Allergens / analysis*
Allergens / immunology*
Cross Reactions*
Enzyme-Linked Immunosorbent Assay / methods*
Fabaceae / immunology*
Food Hypersensitivity / immunology*
Humans
Lupinus / immunology*

Substances

Allergens