Lateral flow assay for brucellosis testing in multiple livestock species

Rajeswari Shome; Triveni Kalleshamurthy; Bibek Ranjan Shome; Swati Sahay; Krithiga Natesan; Rajendra Gulabrao Bambal; Lipi Sairiwal; Nagalingam Mohandoss; Sukhadeo B Barbuddhe

doi:10.1016/j.mimet.2018.03.015

Lateral flow assay for brucellosis testing in multiple livestock species

J Microbiol Methods. 2018 May:148:93-96. doi: 10.1016/j.mimet.2018.03.015. Epub 2018 Mar 30.

Authors

Rajeswari Shome¹, Triveni Kalleshamurthy², Bibek Ranjan Shome², Swati Sahay², Krithiga Natesan², Rajendra Gulabrao Bambal³, Lipi Sairiwal³, Nagalingam Mohandoss², Sukhadeo B Barbuddhe⁴

Affiliations

¹ Indian Council Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Yelahanka, Bengaluru 560064, India. Electronic address: rajeswarishome@gmail.com.
² Indian Council Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Yelahanka, Bengaluru 560064, India.
³ Department of Animal Husbandry, Dairying & Fisheries, Krishi Bhavan, New Delhi 110001, India.
⁴ ICAR-National Research Centre on Meat, Chengicherla, Hyderabad 500092, India.

PMID: 29605524
DOI: 10.1016/j.mimet.2018.03.015

Abstract

Lateral flow assay (LFA) for brucellosis was standardized and evaluated. The test showed high diagnostic sensitivity, specificity and accuracy for diagnosis of brucellosis in bovines, small ruminants and swine. The study emphasized the importance of LFA as a useful, rapid, and easy-to-perform tool for the testing of brucellosis.

Keywords: Brucellosis; Lateral flow assay; Livestock; Serology.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brucellosis / diagnosis
Brucellosis / veterinary*
Cattle
Diagnostic Tests, Routine / methods*
Diagnostic Tests, Routine / standards
Goats
Immunoassay / methods*
Immunoassay / standards
Livestock*
Sensitivity and Specificity
Sheep
Swine