Immunosuppressant Peptide Abu-TGIRIS-Abu-NH2 and its Application for Treatment of Multiple Sclerosis

Valery I Turobov; Viatcheslav N Azev; Alexei B Shevelev; Natalia V Pozdniakova; Yulia K Biryukova; Arkady N Murashev; Valery M Lipkin; Igor P Udovichenko

doi:10.1007/s12668-018-0513-8

Immunosuppressant Peptide Abu-TGIRIS-Abu-NH₂ and its Application for Treatment of Multiple Sclerosis

Bionanoscience. 2018;8(1):484-489. doi: 10.1007/s12668-018-0513-8. Epub 2018 Mar 2.

Authors

Valery I Turobov¹, Viatcheslav N Azev¹, Alexei B Shevelev², Natalia V Pozdniakova³, Yulia K Biryukova², Arkady N Murashev^{1

4}, Valery M Lipkin¹, Igor P Udovichenko^{1

5}

Affiliations

¹ 1 Laboratory of Protein Chemistry, Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospekt Nauki, 6, Pushchino, 142290 Russia.
² 2Emanuel Institute of Biochemical Physics, Moscow, Russia.
³ 3Blokhin Russian Cancer Research Centre, Moscow, Russia.
⁴ 4Pushchino Research Center, Russian Academy of Sciences, Pushchino, Russia.
⁵ 5Pushchino State Institute of Natural Sciences, Pushchino, Russia.

Abstract

Immunosuppressant peptide immunocortin for the first time was described in 1993. It corresponds to residues 11-20 of human Ig heavy chain (conserved motif of V_H domain). There are no data about production of immunocortin by proteolysis of Ig in vivo. Synthetic immunocortin in concentration ~ 10^-9 M suppresses phagocytosis in peritoneal macrophages, ConA-dependent blast transformation of rat lymphocytes, exhibits ACTH-like neurotropic activity and was suggested as a potential drug for treatment of a multiple sclerosis (MS). Here, we report a sequence and method of synthesis of Abu-TGIRIS-Abu-NH₂ (Abu, alpha-aminobutyric acid), an artificial analogue of immunocortin. Biological trials of peritoneally injected Abu-TGIRIS-Abu-NH₂ gave an evidence of its better efficacy versus immunocortin in a test for suppression of the experimental autoimmune encephalomyelitis (EAE) in Dark Agouti (DA) rats.

Keywords: ACTH; Drugs; Immunocortin; Immunosuppressants; Multiple sclerosis; Peptide.