Objective: This study aims to investigate the association between human leucocyte antigen (HLA)-DQB1 allele polymorphisms and the presence dental caries among the Uygur and Han children in Xinjiang.
Methods: HLA-DQB1 allele in the Uygur and Han children with dental caries and healthy control in Xinjiang was tested (n=40) using the polymerase chain reaction-sequence specific primer (PCR-SSP) DNA parting technology.
Results: A total of five specific loci were detected in the HLA-DQB1 locus among the Uygur and Han children. The frequency of the HLA-DQB1*02 allele in the Han group with severe caries (12.5%) was significantly lower than in the control group (32.5%) (P<0.05, OR=0.297). Moreover, the frequency of the HLA-DQB1*05 allele in the Uygur group with severe caries (37.5%) was significantly higher than in the control group (17.5%) (P<0.05, OR=2.829).
Conclusions: Caries susceptibility among the Uygur and Han children in Xinjiang is related to the HLA-DQB1 allele. The HLA-DQB1*02 allele may protect against caries among the Han children, whereas the HLA-DQB1*05 allele may be responsible for the susceptibility of the Uygur children to caries.
目的 探讨人类白细胞抗原（HLA）-DQB1等位基因多态性与新疆维吾尔族和汉族儿童龋病的相关性。方法 采用聚合酶链式反应-序列特异性引物（PCR-SSP）DNA分型技术对新疆维吾尔族和汉族高龋儿童及健康无龋儿童（n=40）进行HLA-DQB1基因分型，研究HLA-DQB1基因多态性与新疆维吾尔族和汉族儿童龋病的相关性。结果 维吾尔族与汉族儿童在HLA-DQB1座位均检出5个特异性基因位点。汉族高龋组患者HLA-DQB1*02等位基因频率（12.5%）显著低于对照组（32.5%），差异有统计学意义（P<0.05，OR=0.297）；维吾尔族高龋组患者HLA-DQB1*05等位基因频率（37.5%）显著高于对照组（17.5%），差异有统计学意义（P<0.05，OR=2.829）。结论 新疆维吾尔族和汉族儿童龋病与HLA-DQB1等位基因多态性具有一定的相关性；HLA-DQB1*02基因可能是新疆汉族儿童龋病的保护因子；而HLA-DQB1*05等位基因是新疆维吾尔族儿童龋病的易感基因。.
Keywords: early childhood caries; genetic polymorphism; human leucocyte antigen.