Abstract
Cell-based immunotherapy for cancer is emerging as an attractive alternative to conventional small-molecule or antibody-based treatment. Due to the characteristics of cell-based therapy, validation of test materials before in vivo administration is required. Here we describe general validation steps for preclinical evaluation of cell-based immunotherapy. We also describe a xenograft model of human colorectal cancer. This model can be used for applied to preclinical evaluation of various cell-based therapy regimens for colorectal cancer treatment.
Keywords:
Cell line; Colorectal cancer; Human tumor xenograft model; Immune cells; Immunotherapy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Culture Techniques / instrumentation
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Cell Culture Techniques / methods
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Cell Line, Tumor
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Cell Survival
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Cell Transplantation / instrumentation
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Cell Transplantation / methods*
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Colorectal Neoplasms / immunology
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Colorectal Neoplasms / therapy*
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Dendritic Cells / immunology
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Dendritic Cells / transplantation
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Flow Cytometry / instrumentation
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Flow Cytometry / methods
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Humans
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Immunotherapy / instrumentation
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Immunotherapy / methods*
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Killer Cells, Natural / immunology
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Killer Cells, Natural / transplantation
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Mice
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Mice, Nude
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Mice, SCID
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T-Lymphocytes / immunology
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T-Lymphocytes / transplantation
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Validation Studies as Topic
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Xenograft Model Antitumor Assays / instrumentation
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Xenograft Model Antitumor Assays / methods*