Isolation and Cultivation of Epidermal (Stem) Cells

Methods Mol Biol. 2019:1879:133-138. doi: 10.1007/7651_2018_132.

Abstract

Recent studies have shown that epidermal stem cells derived from the epidermis of are able to form hair follicles in the presence of hair follicle-inducing cells. Here we describe the method that we have used to isolate and cultivate epidermal stem cells.

Keywords: Cryopreservation; Cultivation; Epidermal stem cells; Isolation.

MeSH terms

  • Animals
  • Epidermal Cells / cytology*
  • Epidermis / physiology
  • Hair Follicle / cytology
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Stem Cells / cytology*