The pH-based fed-batch for lipid production from Trichosporon oleaginosus with crude glycerol

Abstract

In this study, it was found that the optimal pH for the growth of Trichosporon oleaginosus was related to the fermentation medium. A neutral or weak acid pH condition was optimal for the growth of Trichosporon oleaginosus in the extract-peptone-dextrose and wastewater sludge medium. Significant inhibition was observed at neutral pH in the wastewater sludge + crude glycerol medium due to the high soap content of the crude glycerol. By converting the soap to free fatty acid (FFA) at pH 5, the soap inhibition could be prevented. Fed-batch fermentation was employed to produce lipid from Trichosporon oleaginosus at pH 5 controlled by feeding crude glycerol. A remarkably high biomass (65.63 g/L) and lipid (35.79 g/L) concentration were achieved from the pH-based fed-batch fermentation in this study.

Keywords: Crude glycerol; Fed-batch fermentation; Lipid production; Suitable pH.