In Vitro Assessment of Total Phenolic and Flavonoid Contents, Antioxidant and Photoprotective Activities of Crude Methanolic Extract of Aerial Parts of Capnophyllum peregrinum (L.) Lange (Apiaceae) Growing in Algeria

Mostefa Lefahal; Nabila Zaabat; Radia Ayad; El Hani Makhloufi; Lakhdar Djarri; Merzoug Benahmed; Hocine Laouer; Gema Nieto; Salah Akkal

doi:10.3390/medicines5020026

In Vitro Assessment of Total Phenolic and Flavonoid Contents, Antioxidant and Photoprotective Activities of Crude Methanolic Extract of Aerial Parts of Capnophyllum peregrinum (L.) Lange (Apiaceae) Growing in Algeria

Medicines (Basel). 2018 Mar 22;5(2):26. doi: 10.3390/medicines5020026.

Authors

Mostefa Lefahal¹, Nabila Zaabat², Radia Ayad³, El Hani Makhloufi⁴, Lakhdar Djarri⁵, Merzoug Benahmed⁶, Hocine Laouer⁷, Gema Nieto⁸, Salah Akkal⁹

Affiliations

¹ Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine, Constantine 25000, Algeria. mlefahal@gmail.com.
² Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine, Constantine 25000, Algeria. z_nabila2002@yahoo.fr.
³ Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine, Constantine 25000, Algeria. radia.ayad@yahoo.fr.
⁴ Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine, Constantine 25000, Algeria. makhloufi_el_hani@yahoo.fr.
⁵ Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine, Constantine 25000, Algeria. djarri62@gmail.com.
⁶ Department of Chemistry, Faculty of Science, University of Tebessa, Tebessa 12000, Algeria. Riad43200@yahoo.fr.
⁷ Laboratory of Natural Biological Resources Valorization, Faculty of Sciences, University of Setif, Setif 19000, Algeria. hocine_laouer@yahoo.fr.
⁸ Department of Food Technology, Nutrition and Food Science, Faculty of veterinary Sciences, University of Murcia, Campus de Espinardo, Espinardo, 30100 Murcia, Spain. gnieto@um.es.
⁹ Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine, Constantine 25000, Algeria. salah4dz@yahoo.fr.

Abstract

Background:Capnophyllum peregrinum (L.) Lange (Apiaceae) is the unique taxon of capnophyllum genus in Algerian flora. It has never been investigated in regards to its total phenolic and flavonoid contents and antioxidant and photoprotective activities. Methods:C. peregrinum aerial parts extracted with absolute methanol. The total flavonoid and phenolic contents of the extract were evaluated to determine their correlation with the antioxidant and photoprotective activities of the extract. Results: The methanolic extract demonstrated a significant amount of phenolics and flavonoids (74.06 ± 1.23 mg GAE/g, 44.09 ± 2.13 mg QE/g, respectively) and exhibited good antioxidant activity in different systems, especially in 1,1-Diphenyl-2-picrylhydrazyl (DPPH), reducing power and total antioxidant capacity assays. Furthermore the extract showed high photoprotective activity with the sun protection factor (SPF) value = 35.21 ± 0.18. Conclusions: The results of the present study show, that the methanolic extract could be used as a natural sunscreen in pharmaceutics or cosmetic formulations and as a valuable source of natural antioxidants.

Keywords: Capnophyllum peregrinum; antioxidant activity; photoprotective activity.