Metabolic profiling of moulds with laser desorption/ionization mass spectrometry on gold nanoparticle enhanced target

Anal Biochem. 2018 May 15:549:45-52. doi: 10.1016/j.ab.2018.03.016. Epub 2018 Mar 16.

Abstract

Surface-assisted laser desorption/ionization mass spectrometry on gold nanoparticle enhanced target (AuNPET) technique was used for metabolomic analysis and secondary metabolites detection of two mould strains - Aspergillus versicolor and Penicillium chrysogenum in model conditions on microbiological malt extract agar medium. Results obtained with the use of AuNPET-based mass spectrometry technique were compared with traditional matrix-assisted laser desorption/ionization (MALDI) method based on α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB) matrices. Gold nanoparticle enhanced target method enabled effective ionization of microbial cellular extract ingredients without interference from the matrix and also improved calibration of spectra resulting in the detection of much higher amount of characteristic metabolites for studied organisms than MALDI.

Keywords: Aspergillus; Gold nanoparticles; Laser desorption/ionization; Mass spectrometry; Metabolome; Moulds; Penicillium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus / metabolism*
  • Coumaric Acids / metabolism
  • Gentisates / metabolism
  • Gold / chemistry*
  • Metabolomics / methods*
  • Metal Nanoparticles / chemistry*
  • Penicillium chrysogenum / metabolism*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Coumaric Acids
  • Gentisates
  • alpha-cyano-4-hydroxycinnamate
  • Gold