Evolution of echovirus 11 in a chronically infected immunodeficient patient

PLoS Pathog. 2018 Mar 19;14(3):e1006943. doi: 10.1371/journal.ppat.1006943. eCollection 2018 Mar.

Abstract

Deep sequencing was used to determine complete nucleotide sequences of echovirus 11 (EV11) strains isolated from a chronically infected patient with CVID as well as from cases of acute enterovirus infection. Phylogenetic analysis showed that EV11 strains that circulated in Israel in 1980-90s could be divided into four clades. EV11 strains isolated from a chronically infected individual belonged to one of the four clades and over a period of 4 years accumulated mutations at a relatively constant rate. Extrapolation of mutations accumulation curve into the past suggested that the individual was infected with circulating EV11 in the first half of 1990s. Genomic regions coding for individual viral proteins did not appear to be under strong selective pressure except for protease 3C that was remarkably conserved. This may suggest its important role in maintaining persistent infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Biological Evolution*
  • Enterovirus B, Human / classification
  • Enterovirus B, Human / genetics*
  • Enterovirus B, Human / isolation & purification*
  • Enterovirus Infections / virology*
  • Genome, Viral*
  • Genomics / methods
  • Humans
  • Immunocompromised Host*
  • Phylogeny
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*

Substances

  • 3' Untranslated Regions
  • Viral Proteins

Grants and funding

The work was performed using research budgets of the US Food and Drug Administration and the Israeli Ministry of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No outside / grant support was used to fund this research.