Assay for improved detection of antigen-specific immune cells from extrasanguinous fluids

David Schub; Mathias Fousse; Julia Elsäßer; Klaus Faßbender; Urban Sester; Tina Schmidt; Martina Sester

doi:10.1002/eji.201847490

Assay for improved detection of antigen-specific immune cells from extrasanguinous fluids

Eur J Immunol. 2018 Aug;48(8):1412-1414. doi: 10.1002/eji.201847490. Epub 2018 Apr 23.

Authors

David Schub¹, Mathias Fousse², Julia Elsäßer³, Klaus Faßbender², Urban Sester⁴, Tina Schmidt¹, Martina Sester¹

Affiliations

¹ Department of Transplant and Infection Immunology, Saarland University, Homburg, Germany.
² Department of Neurology, Saarland University, Homburg, Germany.
³ Department of Urology and Pediatric Urology, Saarland University, Homburg, Germany.
⁴ Department of Internal Medicine IV, Saarland University, Homburg, Germany.

PMID: 29538819
DOI: 10.1002/eji.201847490

Abstract

In this approach, pre-stained cells from extrasanguinous fluids (ESFs) are stimulated in the presence of blood from the same individual. Thus, blood-derived antigen-presenting cells enable stimulation of both ESF- and blood T cells. Pre-staining allows distinction of T cells from ESF and blood, and simultaneous analysis of antigen-specific T cells in both compartments.

Keywords: Cellular immunity; Cerebrospinal fluid; Extrasanguinous fluid; T cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antigen-Presenting Cells / immunology*
Antigens / immunology*
Cerebrospinal Fluid / cytology
Herpesvirus 3, Human / immunology*
Humans
Immunoassay / methods*
Meningitis, Viral / diagnosis*
Meningitis, Viral / immunology
Meningitis, Viral / virology
Mycobacterium tuberculosis / immunology
Neuritis / immunology
Neuritis / virology
Recurrence
T-Lymphocytes / immunology*
Tuberculosis, Pulmonary / diagnosis
Tuberculosis, Pulmonary / immunology

Substances

Antigens