First description of clonal lineage type II (genotype #1) of Toxoplasma gondii in abortion outbreak in goats

Exp Parasitol. 2018 May:188:21-25. doi: 10.1016/j.exppara.2018.03.008. Epub 2018 Mar 8.

Abstract

The purpose of this study was to perform genotypic characterization and to evaluate the virulence of Toxoplasma gondii obtained from aborted fetuses in an abortion outbreak in goats from northeastern Brazil. Brain samples from 32 fetuses were submitted to mouse bioassay for T. gondii isolation. Two isolates were obtained and subjected to genotypic characterization. Isolate virulence was evaluated using murine model in different doses (from 105 to 101 tachyzoites/mL). In genotyping, both isolates were classified as clonal lineage type II (genotype #1 ToxoDB) and showed to be virulent for mice. This is the first description of genotype #1 in cases of goat abortion, showing the circulation of virulent T. gondii isolate producing reproductive disorders in pregnant goat.

Keywords: Genotyping; Goat; Reproductive disorders; Toxoplasmosis; Virulence.

MeSH terms

  • Abortion, Veterinary / epidemiology
  • Abortion, Veterinary / parasitology*
  • Animals
  • Antibodies, Protozoan / blood
  • Biological Assay / veterinary
  • Brain / embryology
  • Brain / parasitology
  • Brazil / epidemiology
  • Cell Line
  • Chlorocebus aethiops
  • Disease Outbreaks / veterinary*
  • Genotype
  • Genotyping Techniques / veterinary
  • Goat Diseases / epidemiology
  • Goat Diseases / parasitology*
  • Goats
  • Mice
  • Phylogeny
  • Polymerase Chain Reaction / veterinary
  • Polymorphism, Restriction Fragment Length
  • Toxoplasma / classification*
  • Toxoplasma / genetics
  • Toxoplasma / immunology
  • Toxoplasma / pathogenicity
  • Toxoplasmosis, Animal / complications
  • Toxoplasmosis, Animal / epidemiology
  • Toxoplasmosis, Animal / parasitology*
  • Virulence

Substances

  • Antibodies, Protozoan