Abstract
Bioassay-guided fractionation of the methanolic extract from the roots of Cynanchum atratum has resulted in the isolation of three new pregnane glycosides (1-3) along with four known compounds (4-7). Their structures were identified by analysis of the spectroscopic data including extensive 2D NMR. All of the isolates were evaluated for their potential to inhibit the melanin production in α-melanocyte stimulating hormone (α-MSH)-activated B16 melanoma cells. Of these, compounds 4-7 dose-dependently inhibited the melanin production with the IC50 values ranging from 4 μM to 33 μM.
Keywords:
Asclepiadaceae; Cynanchum atratum; Melanogenesis inhibitor; Pregnane glycosides; α-MSH.
Copyright © 2018 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology*
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Cynanchum / chemistry*
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Dose-Response Relationship, Drug
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Drug Screening Assays, Antitumor
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Glycosides / chemistry
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Glycosides / isolation & purification
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Glycosides / pharmacology*
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Humans
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Melanins / antagonists & inhibitors*
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Melanins / biosynthesis
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Melanoma, Experimental / drug therapy*
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Melanoma, Experimental / metabolism
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Melanoma, Experimental / pathology
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Molecular Conformation
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plant Roots / chemistry
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Pregnanes / chemistry
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Pregnanes / isolation & purification
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Pregnanes / pharmacology*
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Structure-Activity Relationship
Substances
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Antineoplastic Agents, Phytogenic
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Glycosides
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Melanins
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Plant Extracts
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Pregnanes
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pregnane glycoside