Electrotaxis: Cell Directional Movement in Electric Fields

Methods Mol Biol. 2018:1749:325-340. doi: 10.1007/978-1-4939-7701-7_23.

Abstract

Electrotaxis plays an important role during embryogenesis, inflammation, wound healing, and tumour metastasis. However, the mechanisms at play during electrotaxis are still poorly understood. Therefore intensive studies on signaling pathways involved in this phenomenon should be carried out. In this chapter, we described an experimental system for studying electrotaxis of Amoeba proteus, mouse embryonic fibroblasts (MEF), Walker carcinosarcoma cells WC256, and bone marrow adherent cells (BMAC).

Keywords: Electric field; Electrotactic chamber; Electrotaxis; Galvanotaxis; Time-lapse videomicroscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Movement / physiology*
  • Electricity*
  • Mice
  • Microscopy, Video
  • Signal Transduction / genetics
  • Signal Transduction / physiology
  • Taxis Response / physiology
  • Time-Lapse Imaging
  • Wound Healing / genetics
  • Wound Healing / physiology