Here, we present different methods for immunoprecipitating membrane proteins of Arabidopsis thaliana root material. We describe two extraction methods for the precipitation either for an integral membrane protein of the endoplasmic reticulum (ER) or a peripheral membrane protein partially localized at the plasma membrane, where we precipitate the protein out of the total membrane as well as total cytosolic fractions.
Keywords: Arabidopsis thaliana; Cross-linked beads; Cytosolic fraction; Immunoprecipitation; Integral membrane proteins; Membrane fraction; Peripheral membrane proteins.