Influence of collecting substrate on the Raman imaging of micron-sized particles

Guillaume Falgayrac; Damian Siepka; Elżbieta A Stefaniak; Guillaume Penel; Sophie Sobanska

doi:10.1016/j.aca.2018.02.015

Influence of collecting substrate on the Raman imaging of micron-sized particles

Anal Chim Acta. 2018 Jul 19:1014:41-49. doi: 10.1016/j.aca.2018.02.015. Epub 2018 Feb 14.

Authors

Guillaume Falgayrac¹, Damian Siepka², Elżbieta A Stefaniak³, Guillaume Penel⁴, Sophie Sobanska⁵

Affiliations

¹ Univ. Lille, Univ. Littoral Côte d'Opale, EA 4490 - PMOI - Physiopathologie des Maladies Osseuses Inflammatoires, F-59000 Lille, France. Electronic address: guillaume.falgayrac@univ-lille2.fr.
² Laboratoire de Spectrochimie Infrarouge et Raman, UMR CNRS 8516, Lille 1 University - Science and Technology, Bat. C5, 59655 Villeneuve d'Ascq Cedex, France; Institut des Sciences Moléculaires, UMR CNRS 5255, University of Bordeaux, 351 cours de la Libération, 33405 Talence, France; Laboratory of Composite and Biomimetic Materials, Centre for Interdisciplinary Research, The John Paul II Catholic University of Lublin, Konstantynów 1J, 20-708 Lublin, Poland.
³ Laboratory of Composite and Biomimetic Materials, Centre for Interdisciplinary Research, The John Paul II Catholic University of Lublin, Konstantynów 1J, 20-708 Lublin, Poland.
⁴ Univ. Lille, Univ. Littoral Côte d'Opale, EA 4490 - PMOI - Physiopathologie des Maladies Osseuses Inflammatoires, F-59000 Lille, France.
⁵ Laboratoire de Spectrochimie Infrarouge et Raman, UMR CNRS 8516, Lille 1 University - Science and Technology, Bat. C5, 59655 Villeneuve d'Ascq Cedex, France; Institut des Sciences Moléculaires, UMR CNRS 5255, University of Bordeaux, 351 cours de la Libération, 33405 Talence, France. Electronic address: sophie.sobanska@u-bordeaux.fr.

PMID: 29523250
DOI: 10.1016/j.aca.2018.02.015

Abstract

The influence of six common substrates on the Raman imaging of micron-sized inorganic aerosol particles was examined. Laboratory-generated single-component particles of calcite (CaCO₃) and mixed particles of calcite (CaCO₃), nitratine (NaNO₃), hematite (Fe₂O₃) and anglesite (PbSO₄) were deposited by cascade impaction on Ag, In, Si, SiO₂, microscope slide and TEM-grid substrates. The spectral contribution of substrates to Raman images of the deposited particles was evaluated by Multivariate Curve Resolution. The shape and intensity of the substrate spectra affect the effectiveness capability of the spectral deconvolution. The substrates were characterized and compared with respect to their effect on the reconstruction of Raman images of aerosol particles. The TEM-grid substrate yielded spatially stable sample measurements with a homogeneous spectral contribution, satisfactory Raman map reconstruction and the potential for application in other techniques (e.g., SEM-EDX).

Keywords: Imaging; Micron-sized; Multivariate curve resolution; Particles; Raman spectroscopy; Substrates.