Analyzing Chemotaxis and Related Behaviors of Azospirillum Brasilense

Curr Protoc Microbiol. 2018 Feb 22:48:3E.3.1-3E.3.11. doi: 10.1002/cpmc.49.

Abstract

Bacteria of the genus A. brasilense are motile and capable of chemotaxis and aerotaxis (taxis in gradient of oxygen) using a single polar flagellum that propels the cells in aqueous environments. Responses to attractants and repellents have been described and spatial gradient assays that permit the visualization of these responses are detailed in this unit. These assays are simple and can be readily implemented with minimum set ups. © 2018 by John Wiley & Sons, Inc.

Keywords: Azospirillum; aerotaxis; chemotaxis; gradient assays; taxis behaviors.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Azospirillum brasilense / genetics
  • Azospirillum brasilense / physiology*
  • Bacteriological Techniques / methods*
  • Chemotaxis*
  • Microscopy / methods*