Growth and Laboratory Maintenance of Paenibacillus larvae

Osama S Mahdi; Nathan A Fisher

doi:10.1002/cpmc.45

Growth and Laboratory Maintenance of Paenibacillus larvae

Curr Protoc Microbiol. 2018 Feb 22:48:9E.1.1-9E.1.6. doi: 10.1002/cpmc.45.

Authors

Osama S Mahdi¹, Nathan A Fisher²

Affiliations

¹ Department of Microbiological Sciences, North Dakota State University, Fargo, North Dakota.
² Southern Research Institute, Frederick, Maryland.

PMID: 29512117
DOI: 10.1002/cpmc.45

Abstract

Paenibacillus larvae is a Gram-positive, spore-forming bacterium and the causative agent of American foulbrood disease (AFB), a highly contagious, fatal disease affecting managed honeybee (Apis mellifera) colonies. As the etiological agent of American foulbrood disease, P. larvae is the most economically significant bacterial pathogen infecting honeybees. This unit includes protocols for the in vitro growth and laboratory maintenance of P. larvae. © 2018 by John Wiley & Sons, Inc.

Keywords: American foulbrood disease (AFB); Paenibacillus larvae; honeybees.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bees / microbiology
Colony Count, Microbial / methods*
Culture Media / metabolism
Paenibacillus larvae / genetics
Paenibacillus larvae / growth & development*
Paenibacillus larvae / metabolism
Preservation, Biological / methods*

Substances

Culture Media