Abstract
Two new sucrose derivatives, namely, belamcanosides A (1) and B (2), together with five other known compounds (3-7), were isolated from the seeds of Belamcanda chinensis (L.) DC. Their structures were identified based on spectroscopic data. Especially, the absolute configurations of fructose and glucose residues in 1 and 2 were assigned by acid hydrolysis, followed by derivatization and gas chromatography (GC) analysis. Among the known compounds, (-)-hopeaphenol (3), (+)-syringaresinol (4), and quercetin (5), were isolated from B. chinensis for the first time. In addition, biological evaluation of 1 and 2 against cholesterol synthesis and metabolism at the gene level was carried out. The results showed that compounds 1 and 2 could regulate the expression of cholesterol synthesis and metabolism-associated genes, including 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), squalene epoxidase (SQLE), low density lipoprotein receptor (LDLR), and sortilin (SORT1) genes in HepG2 cells.
Keywords:
Belamcanda chinensis; GC analysis; HMGCR; LDLR; SORT1; SQLE; belamcanosides A and B.
MeSH terms
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Adaptor Proteins, Vesicular Transport / genetics
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Adaptor Proteins, Vesicular Transport / metabolism
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Cell Survival / drug effects
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Cholesterol / biosynthesis
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Furans / chemistry*
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Furans / isolation & purification
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Furans / pharmacology
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Gene Expression Regulation
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Hep G2 Cells
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Humans
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Hydroxymethylglutaryl CoA Reductases / genetics
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Hydroxymethylglutaryl CoA Reductases / metabolism
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Iris Plant / chemistry*
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Lignans / chemistry*
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Lignans / isolation & purification
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Lignans / pharmacology
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Phenols / chemistry*
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Phenols / isolation & purification
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Phenols / pharmacology
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Plant Extracts / chemistry
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Quercetin / chemistry*
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Quercetin / isolation & purification
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Quercetin / pharmacology
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Receptors, LDL / genetics
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Receptors, LDL / metabolism
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Seeds / chemistry*
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Squalene Monooxygenase / genetics
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Squalene Monooxygenase / metabolism
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Stilbenes / chemistry*
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Stilbenes / isolation & purification
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Stilbenes / pharmacology
Substances
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Adaptor Proteins, Vesicular Transport
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Furans
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LDLR protein, human
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Lignans
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Phenols
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Plant Extracts
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Receptors, LDL
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Stilbenes
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hopeaphenol
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syringaresinol
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Cholesterol
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Quercetin
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HMGCR protein, human
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Hydroxymethylglutaryl CoA Reductases
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Squalene Monooxygenase
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sortilin