Micellar electrokinetic chromatography with laser induced fluorescence detection shows increase of putrescine in erythrocytes of Parkinson's disease patients

L Betancourt; P Rada; L Hernandez; H Araujo; G A Ceballos; L E Hernandez; P Tucci; Z Mari; M De Pasquale; D A Paredes

doi:10.1016/j.jchromb.2018.02.015

Micellar electrokinetic chromatography with laser induced fluorescence detection shows increase of putrescine in erythrocytes of Parkinson's disease patients

J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Apr 1:1081-1082:51-57. doi: 10.1016/j.jchromb.2018.02.015. Epub 2018 Feb 13.

Authors

L Betancourt¹, P Rada², L Hernandez², H Araujo³, G A Ceballos⁴, L E Hernandez², P Tucci⁵, Z Mari⁶, M De Pasquale⁷, D A Paredes⁸

Affiliations

¹ Department of Morphological Sciences, Medical School, University of the Andes, Mérida, Venezuela; Laboratory of Behavioral Physiology, Medical School, University of the Andes, Mérida, Venezuela.
² Laboratory of Behavioral Physiology, Medical School, University of the Andes, Mérida, Venezuela.
³ Department of Neurology, University Hospital, University of Los Andes, Mérida, Venezuela.
⁴ Department of Electrical Engineering, Engineering School, University of the Andes, Mérida, Venezuela.
⁵ Laboratory of Scientific Instrumentation, University of the Andes, Mérida, Venezuela.
⁶ Lou Ruvo Center for Brain Health, Cleveland Clinic Lou Ruvo Center for Brain Health, Las Vegas, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
⁷ Lieber Institute for Brain Development, Johns Hopkins University, Baltimore, MD, USA.
⁸ Lieber Institute for Brain Development, Johns Hopkins University, Baltimore, MD, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Knoebel Institute for Healthy Aging, University of Denver, Denver, CO, USA. Electronic address: daniel.paredes@du.edu.

PMID: 29502029
DOI: 10.1016/j.jchromb.2018.02.015

Abstract

A highly sensitive method was developed to measure putrescine by micellar electrokinetic chromatography with laser induced fluorescence detection with excellent linearity in the 1 nM to 3 μM range. The technique was tested on a drop of blood from Parkinson's disease patients obtained by finger prick. The results showed a statistically significant increase of putrescine in the erythrocytes compared to controls and a non-significant increase in plasma. This high level of putrescine does not constitute by itself proof that putrescine and polyamines are directly related to Parkinson's disease. However, the present results and several others addressed in the discussion suggest that these compounds might be causally involved in the pathophysiology of Parkinson's disease. In addition, the analytical method reported here may help to find new biomarkers for many diseases including Parkinson's disease.

Keywords: Biomarker; Capillary zone electrophoresis; Fluorescence detection; Micellar electrokinetic chromatography; Parkinson's disease; Polyamines; Putrescine.

MeSH terms

Aged
Aged, 80 and over
Biomarkers / blood
Chromatography, Micellar Electrokinetic Capillary / methods*
Erythrocytes / chemistry*
Humans
Limit of Detection
Linear Models
Middle Aged
Parkinson Disease / blood*
Putrescine / blood*
Reproducibility of Results
Spectrometry, Fluorescence / methods*

Substances

Biomarkers
Putrescine