An extracellular yellow laccase from white rot fungus Trametes sp. F1635 and its mediator systems for dye decolorization

Shou-Nan Wang; Qing-Jun Chen; Meng-Juan Zhu; Fei-Yang Xue; Wei-Cong Li; Tian-Jian Zhao; Guang-Dong Li; Guo-Qing Zhang

doi:10.1016/j.biochi.2018.02.015

An extracellular yellow laccase from white rot fungus Trametes sp. F1635 and its mediator systems for dye decolorization

Biochimie. 2018 May:148:46-54. doi: 10.1016/j.biochi.2018.02.015. Epub 2018 Mar 5.

Authors

Shou-Nan Wang¹, Qing-Jun Chen², Meng-Juan Zhu³, Fei-Yang Xue¹, Wei-Cong Li¹, Tian-Jian Zhao¹, Guang-Dong Li¹, Guo-Qing Zhang⁴

Affiliations

¹ Key Laboratory of Urban Agriculture (North China) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China.
² Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
³ Department of Fungal Resource, Shandong Agricultural University, Taian, Shandong 271018, China.
⁴ Key Laboratory of Urban Agriculture (North China) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China. Electronic address: zhanggqbua@163.com.

PMID: 29501482
DOI: 10.1016/j.biochi.2018.02.015

Abstract

A novel extracellular laccase was purified from fermentation broth of the white rot fungus Trametes sp. F1635 by a three-step protocol including two consecutive ion-exchange chromatography steps on DEAE-Sepharose and SP-Sepharose, and a final gel-filtration on Superdex 75. The purified laccase (TsL) was a monomeric protein with the molecular mass of 64.8 kDa. It demonstrated high oxidation activity of 4.00 × 10⁴ U/mg towards ABTS. Its N-terminal amino acid sequence was AIGPVADLTIINNAV which was unique and sharing high similarity of other fungal laccases. TsL was a yellow laccase based on absorption spectrum analysis. It demonstrated an acidic pH optimum of 2.6 and temperature optimum of 50 °C towards ABTS. The K_m and V_max values towards ABTS were estimated to 18.58 μM and 1.35 μmol/min, respectively. TsL manifested effective decolorization activity towards eriochrome black T (EBT), remazol brilliant blue R (RBBR), malachite green (MG), and eriochrome black T (EBT) (over 60%). Violuric acid (VA) and acetosyringone (AS) were the optimal mediators for the laccase in dye decolorization. Results suggest that TsL demonstrates great potential for dye decolorization and water treatment.

Keywords: Dye decolorization; Laccase; Laccase-mediator system; Purification; Trametes.

MeSH terms

Amino Acid Sequence
Color
Coloring Agents / metabolism*
Extracellular Space / enzymology*
Fermentation
Hydrogen-Ion Concentration
Kinetics
Laccase / chemistry
Laccase / metabolism*
Molecular Weight
Temperature
Trametes / cytology*
Trametes / enzymology

Substances

Coloring Agents
Laccase