Sensitive immunoassay of von Willebrand factor based on fluorescence resonance energy transfer between graphene quantum dots and Ag@Au nanoparticles

Abstract

Graphene quantum dots (GQDs) and core-shell Ag@Au nanoparticles (Ag@Au NPs) were synthetized and they were characterized by transmission electron microscope and X-ray photoelectron spectra, respectively. Von Willebrand factor antibody (vWF Ab) was bound on Ag@Au NPs to construct Ag@Au-Ab nanocomposites (Ag@Au-Ab NCs). The fluorescence of GQDs could be effectively quenched by the prepared nanocomposites owing to fluorescence resonance energy transfer (FRET). The immunoreaction between vWF and Ag@Au-Ab NCs resulted in the declined FRET efficiency and a degree of fluorescence recovery of GQDs. The fluorescence intensity change was found to be proportional to the logarithm of the vWF concentration in the range of 0.1 pg mL^-1-10 ng mL^-1 with a detection limit of 30 fg mL^-1. The proposed fluorescence sensor was employed to investigate the relationship between the release of vWF and the oxidation-injury degree of vascular endothelial cells. The experimental results indicate that the vWF content in the growth medium was enhanced and the cell injury was intensified when the contact time of the cells with H₂O₂ was increased.

Keywords: Ag@Au nanoparticles; Fluorescence resonance energy transfer; Graphene quantum dots; Immunoassay; von Willebrand factor.