A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains

Rafael Lucas Muniz Guedes; Carla Monadeli Filgueira Rodrigues; Nicolas Coatnoan; Alain Cosson; Fabiano Antonio Cadioli; Herakles Antonio Garcia; Alexandra Lehmkuhl Gerber; Rosangela Zacarias Machado; Paola Marcella Camargo Minoprio; Marta Maria Geraldes Teixeira; Ana Tereza Ribeiro de Vasconcelos

doi:10.1016/j.ygeno.2018.02.017

A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains

Genomics. 2019 May;111(3):407-417. doi: 10.1016/j.ygeno.2018.02.017. Epub 2018 Feb 27.

Affiliations

¹ Laboratório Nacional de Computação Científica (LNCC), Av. Getúlio Vargas, 333, Petrópolis, RJ, Brazil; Grupo Hermes Pardini, Setor de Pesquisa e Desenvolvimento, Vespasiano, MG, Brazil. Electronic address: rmguedes@lncc.br.
² Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-900, Brazil.
³ Trypanosomatids Infectious Processes Laboratory, Department of Infection and Epidemiology, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, France.
⁴ Departamento Clínica, Cirurgia e Reprodução Animal, Faculdade de Odontologia e Curso de Medicina Veterinária, Universidade Estadual Paulista - UNESP, Araçatuba, SP, Brazil.
⁵ Laboratório Nacional de Computação Científica (LNCC), Av. Getúlio Vargas, 333, Petrópolis, RJ, Brazil.
⁶ Laboratório de Immnoparasitologia, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (UNESP), Campus Jaboticabal, Jaboticabal, SP, Brazil.

PMID: 29499243
DOI: 10.1016/j.ygeno.2018.02.017

Abstract

Trypanosoma vivax is a parasite widespread across Africa and South America. Immunological methods using recombinant antigens have been developed aiming at specific and sensitive detection of infections caused by T. vivax. Here, we sequenced for the first time the transcriptome of a virulent T. vivax strain (Lins), isolated from an outbreak of severe disease in South America (Brazil) and performed a computational integrated analysis of genome, transcriptome and in silico predictions to identify and characterize putative linear B-cell epitopes from African and South American T. vivax. A total of 2278, 3936 and 4062 linear B-cell epitopes were respectively characterized for the transcriptomes of T. vivax LIEM-176 (Venezuela), T. vivax IL1392 (Nigeria) and T. vivax Lins (Brazil) and 4684 for the genome of T. vivax Y486 (Nigeria). The results presented are a valuable theoretical source that may pave the way for highly sensitive and specific diagnostic tools.

Keywords: Bioinformatics; Bloodstream forms; Linear B-cell epitopes; Transcriptomics; Trypanosoma vivax.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Epitopes, B-Lymphocyte / genetics*
Epitopes, B-Lymphocyte / immunology
Goats
Transcriptome*
Trypanosoma / genetics*
Trypanosoma / immunology

Substances

Epitopes, B-Lymphocyte