SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

Y G Ermakova; V V Pak; Y A Bogdanova; A A Kotlobay; I V Yampolsky; A G Shokhina; A S Panova; R A Marygin; D B Staroverov; D S Bilan; H Sies; V V Belousov

doi:10.1039/c7cc08740c

SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

Chem Commun (Camb). 2018 Mar 15;54(23):2898-2901. doi: 10.1039/c7cc08740c.

Authors

Y G Ermakova¹, V V Pak, Y A Bogdanova, A A Kotlobay, I V Yampolsky, A G Shokhina, A S Panova, R A Marygin, D B Staroverov, D S Bilan, H Sies, V V Belousov

Affiliation

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia. belousov@ibch.ru.

PMID: 29497715
DOI: 10.1039/c7cc08740c

Abstract

We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pK_a, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.