The New Structure of Core Oligosaccharide Presented by Proteus penneri 40A and 41 Lipopolysaccharides

Int J Mol Sci. 2018 Feb 28;19(3):676. doi: 10.3390/ijms19030676.

Abstract

The new type of core oligosaccharide in Proteus penneri 40A and 41 lipopolysaccharides has been investigated by ¹H and 13C NMR spectroscopy, electrospray ionization mass spectrometry and chemical methods. Core oligosaccharides of both strains were chosen for structural analysis based on the reactivity of LPSs with serum against P. penneri 40A core oligosaccharide-diphtheria toxoid conjugate. Structural analyses revealed that P. penneri 40A and 41 LPSs possess an identical core oligosaccharide.

Keywords: ESI MS; NMR spectroscopy; Proteus penneri; anti-conjugate serum; core oligosaccharide; lipopolysaccharide.

MeSH terms

  • Antigens, Bacterial / chemistry*
  • Antigens, Bacterial / immunology
  • Immune Sera / immunology
  • Lipopolysaccharides / chemistry*
  • Lipopolysaccharides / immunology
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Oligosaccharides / chemistry*
  • Oligosaccharides / immunology
  • Proteus penneri / chemistry*
  • Proteus penneri / immunology
  • Spectrometry, Mass, Electrospray Ionization
  • Structure-Activity Relationship

Substances

  • Antigens, Bacterial
  • Immune Sera
  • Lipopolysaccharides
  • Oligosaccharides