Effect of long-term treatment with classical neuroleptics on NPQ/spexin, kisspeptin and POMC mRNA expression in the male rat amygdala

Artur Pałasz; Marcelina Pałka; Łukasz Filipczyk; Itiana Castro Menezes; Ewa Rojczyk; John J Worthington; Aneta Piwowarczyk-Nowak; Marek Krzystanek; Ryszard Wiaderkiewicz

doi:10.1007/s00702-018-1868-2

Effect of long-term treatment with classical neuroleptics on NPQ/spexin, kisspeptin and POMC mRNA expression in the male rat amygdala

J Neural Transm (Vienna). 2018 Jul;125(7):1099-1105. doi: 10.1007/s00702-018-1868-2. Epub 2018 Feb 27.

Authors

Artur Pałasz¹, Marcelina Pałka², Łukasz Filipczyk², Itiana Castro Menezes³, Ewa Rojczyk⁴, John J Worthington⁵, Aneta Piwowarczyk-Nowak⁶, Marek Krzystanek⁷, Ryszard Wiaderkiewicz²

Affiliations

¹ Department of Histology, School of Medicine in Katowice, Medical University of Silesia, ul. Medyków 18, 40-752, Katowice, Poland. apalasz@sum.edu.pl.
² Department of Histology, School of Medicine in Katowice, Medical University of Silesia, ul. Medyków 18, 40-752, Katowice, Poland.
³ Department of Neurosciences and Behaviour, Faculty of Medicine, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, São Paulo, 14049-900, Brazil.
⁴ Department of Descriptive and Topographic Anatomy, School of Medicine with Division of Dentistry in Zabrze, Medical University of Silesia, ul. Jordana 19, 41-808, Zabrze, Poland.
⁵ Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, LA1 4YQ, UK.
⁶ Department of Anatomy, School of Medicine in Katowice, Medical University of Silesia, ul. Medyków 18, 40-752, Katowice, Poland.
⁷ Department and Clinic of Psychiatric Rehabilitation, School of Medicine in Katowice, Medical University of Silesia, ul. Ziolowa 45/47, 40-635, Katowice, Poland.

Abstract

Neuroleptics modulate the expression level of some regulatory neuropeptides in the brain. However, if these therapeutics influence the peptidergic circuits in the amygdala remains unclear. This study specifies the impact profile of the classical antipsychotic drugs on mRNA expression of the spexin/NPQ, kisspeptin-1 and POMC in the rat amygdala. Animals were treated with haloperidol and chlorpromazine for 28 days prior to transcript quantification via qPCR. Haloperidol and chlorpromazine induced a change in the expression of all neuropeptides analyzed. Both drugs led to the decrease of Kiss-1 expression, whereas in POMC and spexin/NPQ their up-regulation in the amygdala was detected. These modulating effects on may represent alternative, so far unknown mechanisms, of classical antipsychotic drugs triggering pharmacological responses.

Keywords: Amygdala; Chlorpromazine; Haloperidol; Kisspeptin; POMC; Spexin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amygdala / drug effects*
Amygdala / metabolism
Animals
Antipsychotic Agents / pharmacology*
Gene Expression / drug effects
Kisspeptins / biosynthesis
Kisspeptins / drug effects*
Male
Peptide Hormones / biosynthesis
Peptide Hormones / drug effects*
Pro-Opiomelanocortin / biosynthesis
Pro-Opiomelanocortin / drug effects*
RNA, Messenger / analysis
RNA, Messenger / biosynthesis
Rats
Rats, Sprague-Dawley

Substances

Antipsychotic Agents
Kiss1 protein, rat
Kisspeptins
Peptide Hormones
RNA, Messenger
SPX protein, rat
Pro-Opiomelanocortin