Aflatoxins in sunflower seeds and unrefined sunflower oils from Singida, Tanzania

Food Addit Contam Part B Surveill. 2018 Sep;11(3):161-166. doi: 10.1080/19393210.2018.1443519. Epub 2018 Mar 16.

Abstract

A total of 61 samples comprising sunflower seeds (40) and unrefined sunflower oils (21) samples collected randomly from Singida, Tanzania were analysed by Reverse Phase-high performance liquid chromatography (RP-HPLC). 15% (6/40) of the seed samples were contaminated with aflatoxin B1 ranging from limit of detection (LOD) to 218 ng g-1 with three of them exceeding the European Commission/European Union (EC/EU) and Tanzania Bureau of Standards (TBS)/Tanzania Food and Drug Authority (TFDA) maximum limits of 2 ng g-1 for AFB1 in oilseeds. The levels of total aflatoxins (AFT) in seeds ranged from LOD to 243 ng g-1. Other aflatoxins, except AFG2, were also detected. For the unrefined sunflower oils, the levels of AFB1 ranged from LOD to 2.56 ng mL-1. About 80.9% (17/21) of the analysed oil samples contained AFB1 of which 17.65% (3/17) exceeded the EC/EU and TBS/TFDA maximum limits of 2 ng mL-1. Other aflatoxins were also detected in the oils. The measured levels indicate there is a need for food quality education among food processors.

Keywords: Aflatoxin B1; B2; G1; G2; Singida; Tanzania; sunflower seeds; total aflatoxin; unrefined sunflower oils.

Publication types

  • Validation Study

MeSH terms

  • Aflatoxin B1 / analysis
  • Aflatoxin B1 / isolation & purification
  • Aflatoxins / analysis*
  • Aflatoxins / isolation & purification
  • Analytic Sample Preparation Methods
  • Chromatography, High Pressure Liquid
  • Crops, Agricultural / chemistry
  • Crops, Agricultural / growth & development
  • Dietary Fats, Unsaturated / analysis*
  • Dietary Fats, Unsaturated / standards
  • Food Contamination*
  • Food Inspection / methods*
  • Food Storage / standards
  • Helianthus / chemistry*
  • Helianthus / growth & development
  • Humans
  • Limit of Detection
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Reproducibility of Results
  • Seeds / chemistry*
  • Seeds / growth & development
  • Spectrometry, Fluorescence
  • Sunflower Oil / chemistry*
  • Sunflower Oil / standards
  • Tanzania

Substances

  • Aflatoxins
  • Dietary Fats, Unsaturated
  • Plant Extracts
  • Sunflower Oil
  • aflatoxin G1
  • aflatoxin B2
  • Aflatoxin B1