Effects of nicosulfuron on growth, oxidative damage, and the ascorbate-glutathione pathway in paired nearly isogenic lines of waxy maize (Zea mays L.)

Abstract

Nicosulfuron is a postemergence herbicide used for weed control in maize fields (Zea mays L.). We used the pair of nearly isogenic inbred lines, SN509-R (nicosulfuron resistant) and SN509-S (nicosulfuron sensitive), to study the effect of nicosulfuron on growth, oxidative stress, and the ascorbate-glutathione (AA-GSH) cycle in waxy maize seedlings. Nicosulfuron treatment was applied when the fourth leaves were fully developed and the obtained effects were compared to water treatment as control. After nicosulfuron treatment, compared to SN509-R, the death of SN509-S might be associated with increased oxidative stress, since higher O₂^- and H₂O₂ accumulations were observed in SN509-S. This in turn might have caused severe damage to lipids and proteins, thus reducing membrane stability. These effects were exacerbated with increasing exposure time. After nicosulfuron treatment, ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, glutathione reductase, and guaiacol peroxidase of SN509-S were significantly lower than those of SN509-R. Compared to SN509-R, dehydroascorbate content, glutathione (GSH) content, and GSH to glutathione disulphide ratios significantly declined with increasing exposure time in SN509-S. Our results suggest that the rapid degradation of nicosulfuron in SN509-R results in only a small and transient increase in reactive oxygen species (ROS). In contrast, in SN509-S, reduced nicosulfuron degradation leads to increase ROS, while at the same time, the AA-GSH pathway is not activated.

Keywords: Ascorbate-glutathione cycle; Nicosulfuron; Oxidative stress; Waxy maize.