Abstract
The role of AMPK in angiogenesis can be studied using in vitro and in vivo assays. The endothelial spheroid assay is a robust three-dimensional in vitro test, which allows investigation of tubular morphogenesis by integrating cell-cell as well as cell-matrix interactions. The Matrigel plug assay validates the process of angiogenesis in vivo and allows studies in genetically modified mice. Here, we give a detailed description of both assays and their application in AMPK research.
Keywords:
Angiogenesis; Matrigel plug assay; Spheroid assay; Sprouting; Tube formation.
MeSH terms
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AMP-Activated Protein Kinases / genetics
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AMP-Activated Protein Kinases / metabolism
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AMP-Activated Protein Kinases / physiology*
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Animals
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Cell Migration Assays / instrumentation
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Cell Migration Assays / methods
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Cell Proliferation
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Cells, Cultured
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Collagen*
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Drug Combinations
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Human Umbilical Vein Endothelial Cells
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Humans
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Laminin*
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Mice
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Mice, Knockout
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Morphogenesis / physiology
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Neovascularization, Physiologic / physiology*
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Proteoglycans*
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RNA, Small Interfering / metabolism
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Spheroids, Cellular
Substances
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Drug Combinations
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Laminin
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Proteoglycans
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RNA, Small Interfering
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matrigel
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Collagen
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AMP-Activated Protein Kinases