Expression of CD9 and CD81 in bovine germinal vesicle oocytes after vitrification followed by in vitro maturation

Yongshun Ma; Bo Pan; Haoxuan Yang; Izhar Hyder Qazi; Zhenzheng Wu; Changjun Zeng; Ming Zhang; Qingyong Meng; Guangbin Zhou

doi:10.1016/j.cryobiol.2018.02.011

Expression of CD9 and CD81 in bovine germinal vesicle oocytes after vitrification followed by in vitro maturation

Cryobiology. 2018 Apr:81:206-209. doi: 10.1016/j.cryobiol.2018.02.011. Epub 2018 Feb 22.

Authors

Yongshun Ma¹, Bo Pan¹, Haoxuan Yang¹, Izhar Hyder Qazi², Zhenzheng Wu¹, Changjun Zeng¹, Ming Zhang¹, Qingyong Meng³, Guangbin Zhou⁴

Affiliations

¹ Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China.
² Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China; Department of Veterinary Anatomy & Histology, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand 67210, Sindh, Pakistan.
³ State Key Laboratory of AgroBiotechnology, China Agricultural University, Beijing 100193, PR China.
⁴ Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China. Electronic address: zguangbin@sicau.edu.cn.

PMID: 29476719
DOI: 10.1016/j.cryobiol.2018.02.011

Abstract

The present study aimed to investigate the effect of vitrification on the expression of fertilization related genes (CD9 and CD81) and DNA methyl transferases (DNMT1 and DNMT3b) in bovine germinal vesicle (GV) oocytes and their resulting metaphase Ⅱ (MⅡ) stages after in vitro maturation culture. GV oocytes were vitrified using the open-pulled straw method; after warming, they were cultured in vitro. The vitrified-warmed GV oocytes and more developed MII oocytes were used to calculate the maturation rates (first polar body extrusion under a stereomicroscopy), and to detect mRNA expression (qRT-PCR). Fresh GV oocytes and their in vitro-derived MII oocytes served as controls. The results showed that both the maturation rate (54.23% vs. 42.93%) and the relative abundance of CD9 mRNA decreased significantly (p < 0.05) in bovine GV oocytes after vitrification, but the expression of CD81 and DNMT3b increased significantly. After in vitro maturation of vitrified GV oocytes, the resulting MII oocytes showed lower (p < 0.05) mRNA expression of genes (CD9, CD81, DNMT1 and DNMT3b) when compared to the control group (MII oocytes). Altogether, vitrification decreased the maturation rate of bovine GV oocytes and changed the expression of fertilization related genes and DNA methyl transferases during in vitro maturation.

Keywords: Bovine GV oocyte; CD81; CD9; In vitro maturation; Vitrification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cryopreservation / methods*
Female
Oocytes / drug effects
Oocytes / metabolism*
Oogenesis / drug effects
Oogenesis / physiology*
Tetraspanin 28 / biosynthesis*
Tetraspanin 29 / biosynthesis*
Vitrification*

Substances

Tetraspanin 28
Tetraspanin 29