Abstract
Intravital two-photon microscopy is a powerful imaging tool for investigating various biological processes in live animals. This chapter describes an overview of intravital imaging of the rodent kidney including animal surgery, characteristics of renal tubular autofluorescence, in vivo use of fluorescent probes, and renal immune-cell tracking.
Keywords:
Intravital two-photon microscopy; Kidney; Oxidative stress; Renal immune cell imaging.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cell Tracking / methods*
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Endotoxins / pharmacology
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Fluorescent Dyes / metabolism
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Intravital Microscopy / methods*
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Kidney / cytology*
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Kidney / drug effects
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Kidney / surgery
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Membrane Potential, Mitochondrial
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Mice
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Mice, Inbred C57BL
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Microscopy, Fluorescence, Multiphoton / methods*
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Oxidative Stress
Substances
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Endotoxins
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Fluorescent Dyes