Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System During Neuroinflammation and Neurodegeneration

Marina Dukhinova; Ekaterina Kopeikina; Eugene D Ponomarev

doi:10.1007/978-1-4939-7680-5_10

Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System During Neuroinflammation and Neurodegeneration

Methods Mol Biol. 2018:1745:167-177. doi: 10.1007/978-1-4939-7680-5_10.

Authors

Marina Dukhinova¹, Ekaterina Kopeikina¹, Eugene D Ponomarev²

Affiliations

¹ School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China.
² School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China. eponomarev@cuhk.edu.hk.

PMID: 29476469
DOI: 10.1007/978-1-4939-7680-5_10

Abstract

The resident macrophages of the central nervous system (CNS), also known as microglia, and blood-derived macrophages play an important role in the functional activity of the normal CNS, as well as in the development of neuroinflammation during various neurodegenerative disorders. Microglia and macrophages represent heterogeneous populations, which can modulate CNS environment and have different effects on neuronal regeneration. In this chapter, the main features of microglial and macrophage subsets and current methods for investigation of their heterogeneity will be discussed.

Keywords: Macrophages; Microglia; Neurodegeneration; Neuroinflammation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers
Central Nervous System / metabolism*
Central Nervous System / pathology
Flow Cytometry* / methods
Inflammation / diagnosis
Inflammation / metabolism
Macrophages / metabolism*
Microglia / metabolism*
Myeloid Cells / metabolism
Neurodegenerative Diseases / diagnosis
Neurodegenerative Diseases / metabolism

Substances

Biomarkers