Modeling Transient Focal Ischemic Stroke in Rodents by Intraluminal Filament Method of Middle Cerebral Artery Occlusion

Mary Susan Lopez; Raghu Vemuganti

doi:10.1007/978-1-4939-7526-6_9

Modeling Transient Focal Ischemic Stroke in Rodents by Intraluminal Filament Method of Middle Cerebral Artery Occlusion

Methods Mol Biol. 2018:1717:101-113. doi: 10.1007/978-1-4939-7526-6_9.

Authors

Mary Susan Lopez¹, Raghu Vemuganti²

Affiliations

¹ Department of Neurosurgery, University of Wisconsin-Madison, Madison, WI, USA.
² Department of Neurosurgery, University of Wisconsin-Madison, Madison, WI, USA. vemuganti@neurosurgery.wisc.edu.

PMID: 29468587
DOI: 10.1007/978-1-4939-7526-6_9

Abstract

The middle cerebral artery occlusion (MCAO) model is widely used for inducing a focal cerebral ischemic insult (stroke) in rodents. Here, we describe a method for transient MCAO technique without craniotomy in both mice and rats. In our laboratory, this technique yields consistent secondary brain damage that evolves over a period of 3-7 days of reperfusion after transient MCAO. We also describe the methods for analyzing postischemic motor dysfunction and infarct volume in rodents subjected to transient MCAO.

Keywords: Beam-walk test; Focal cerebral ischemia; Infarct volume; Middle cerebral artery occlusion; Rotarod test; Sticker removal test; Transcardiac perfusion.

Publication types

Review

MeSH terms

Animals
Disease Models, Animal
Humans
Infarction, Middle Cerebral Artery* / metabolism
Infarction, Middle Cerebral Artery* / pathology
Infarction, Middle Cerebral Artery* / physiopathology
Mice
Motor Disorders* / metabolism
Motor Disorders* / pathology
Motor Disorders* / physiopathology
Rats
Stroke* / metabolism
Stroke* / pathology
Stroke* / physiopathology