Abstract
Cold temperature blocks used to synchronize protein trafficking inhibit GBF1 function, leading to a decrease in ARF1-GTP levels and mislocalization of the ARF1 effector golgin-160. Several other, but not all, Golgi proteins including ARL1 also mislocalize. ARF1 activity and golgin-160 localization require more than 30 min to recover from these blocks.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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ADP-Ribosylation Factor 1 / genetics
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ADP-Ribosylation Factor 1 / metabolism*
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Autoantigens / genetics
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Autoantigens / metabolism
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Cold Temperature
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Golgi Matrix Proteins / genetics
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Golgi Matrix Proteins / metabolism
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Guanine Nucleotide Exchange Factors / antagonists & inhibitors*
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Guanine Nucleotide Exchange Factors / metabolism
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HeLa Cells
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Humans
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Protein Transport*
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trans-Golgi Network / metabolism*
Substances
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Autoantigens
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GBF1 protein, human
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GOLGA3 protein, human
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Golgi Matrix Proteins
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Guanine Nucleotide Exchange Factors
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ADP-Ribosylation Factor 1