Commonly used trafficking blocks disrupt ARF1 activation and the localization and function of specific Golgi proteins

Mol Biol Cell. 2018 Apr 15;29(8):937-947. doi: 10.1091/mbc.E17-11-0622. Epub 2018 Mar 30.

Abstract

Cold temperature blocks used to synchronize protein trafficking inhibit GBF1 function, leading to a decrease in ARF1-GTP levels and mislocalization of the ARF1 effector golgin-160. Several other, but not all, Golgi proteins including ARL1 also mislocalize. ARF1 activity and golgin-160 localization require more than 30 min to recover from these blocks.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • ADP-Ribosylation Factor 1 / genetics
  • ADP-Ribosylation Factor 1 / metabolism*
  • Autoantigens / genetics
  • Autoantigens / metabolism
  • Cold Temperature
  • Golgi Matrix Proteins / genetics
  • Golgi Matrix Proteins / metabolism
  • Guanine Nucleotide Exchange Factors / antagonists & inhibitors*
  • Guanine Nucleotide Exchange Factors / metabolism
  • HeLa Cells
  • Humans
  • Protein Transport*
  • trans-Golgi Network / metabolism*

Substances

  • Autoantigens
  • GBF1 protein, human
  • GOLGA3 protein, human
  • Golgi Matrix Proteins
  • Guanine Nucleotide Exchange Factors
  • ADP-Ribosylation Factor 1