PTEN expression in astrocytic processes after spinal cord injury

T V Povysheva; Y O Mukhamedshina; A A Rizvanov; Y A Chelyshev

doi:10.1016/j.mcn.2018.02.008

PTEN expression in astrocytic processes after spinal cord injury

Mol Cell Neurosci. 2018 Apr:88:231-239. doi: 10.1016/j.mcn.2018.02.008. Epub 2018 Feb 15.

Authors

T V Povysheva¹, Y O Mukhamedshina², A A Rizvanov³, Y A Chelyshev⁴

Affiliations

¹ Department of Histology, Cytology and Embryology, Kazan State Medical University, Kazan, Tatarstan, Russia.
² Department of Histology, Cytology and Embryology, Kazan State Medical University, Kazan, Tatarstan, Russia; OpenLab Gene and Cell Technologies, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Tatarstan, Russia. Electronic address: yana.k-z-n@mail.ru.
³ OpenLab Gene and Cell Technologies, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Tatarstan, Russia.
⁴ Department of Histology, Cytology and Embryology, Kazan State Medical University, Kazan, Tatarstan, Russia; Department of Medical Physics, Institute of Physics, Kazan (Volga Region) Federal University, Kazan, Tatarstan, Russia.

PMID: 29454667
DOI: 10.1016/j.mcn.2018.02.008

Abstract

The role of the Rho/ROCK/PTEN signaling pathway in the regulation of astrocyte function for consolidation/stabilization of the synapse has not been thoroughly studied. In this study, the expression of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in GFAP-positive astrocytic processes in the ventral horns (VH) of the rat spinal cord has been evaluated in the normal condition and in a delayed period (30 days) after dosed contusion spinal cord injury (SCI) in caudal thoracic segments. In intact rats and at 30 days post-injury (dpi), semi-quantitative immunohistochemical analysis showed that there is approximately 2 folds less synaptophysin reactivity in the motoneuron perikarya than outside the perikarya, i.e., on dendritic spines, in the VH area. At 30 dpi, the square occupied by synaptophysin reactivity on the motoneuron perikarya and dendritic spines decreased ~2.4 and ~2.1 folds, respectively. Western blotting of the postsynaptic density protein 95 (PSD95) showed a decreased amount in the area of injury of ~3 folds at 30 dpi. Expression of GFAP in the astrocytic processes around the synaptophysin spots (APAS) was less than in the astrocytic processes that were located at distance from the synapses (APFS) both in the intact and SCI groups. In the APAS, the expression level of PTEN increased significantly after SCI. In these astrocytic processes, the PTEN expression level was significantly higher than in the APFS for both the intact and SCI rats. In the intact spinal cord, different PTEN expression levels were detected both in APAS and APFS. This may be due to the varying degree of integration of PTEN in the membrane compartment of astrocyte stem processes and possibly the increased delivery of PTEN from the GFAP-positive stem into fine GFAP-negative peripheral processes. The observed shifts after SCI reflect the imbalance in the mechanisms of synaptic plasticity after injury. Thus, strategies that have been developed for the deletion or knockdown of the PTEN gene are quite promising.

Keywords: Astrocytic processes; GFAP; PTEN; Spinal cord injury; Synaptophysin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Astrocytes / metabolism*
Disease Models, Animal
Glial Fibrillary Acidic Protein / metabolism
Motor Neurons / metabolism
Neuronal Plasticity / physiology
PTEN Phosphohydrolase / metabolism*
Rats
Spinal Cord / metabolism*
Spinal Cord Injuries / metabolism*

Substances

GFAP protein, rat
Glial Fibrillary Acidic Protein
PTEN Phosphohydrolase
Pten protein, rat