The fine balance between mutualism and antagonism in the Epichloë festucae-grass symbiotic interaction

Abstract

Epichloë endophytes form mutualistic symbiotic associations with aerial tissues of temperate grasses. Intercalary growth of hyphae within the leaves enables fungal growth to be synchronized with host leaf growth, leading to formation of a highly structured and tightly regulated symbiotic network. Mutations in fungal genes that disrupt cell-cell fusion and other key signalling pathways lead to an antagonistic interaction characterized by unregulated growth of endophytic hyphae and detrimental effects on host growth. Transcriptome analysis of these mutant associations provides key insights into the regulation of the symbiosis. In nature a similar switch in growth occurs when hyphae transition into the sexual cycle forming stromata that abort host inflorescences. Endophyte infection of the grass host leads to a major reprogramming of host metabolism and alters host development. Changes in endophyte cell wall structure and the repertoire of effectors secreted into the host apoplast accompany establishment of a mutualistic interaction within the leaves.