Screening of binding proteins that interact with Chinese sacbrood virus VP3 capsid protein in Apis cerana larvae cDNA library by the yeast two-hybrid method

Virus Res. 2018 Mar 15:248:24-30. doi: 10.1016/j.virusres.2018.02.006. Epub 2018 Feb 13.

Abstract

Chinese sacbrood virus (CSBV) causes larval death and apiary collapse of Apis cerana. VP3 is a capsid protein of CSBV but its function is poorly understood. To determine the function of VP3 and screen for novel binding proteins that interact with VP3, we conducted yeast two-hybrid screening, glutathione S-transferase pull-down, and co-immunoprecipitation assays. Galectin (GAL) is a protein involved in immune regulation and host-pathogen interactions. The yeast two-hybrid screen implicated GAL as a major VP3-binding candidate. The assays showed that the VP3 interacted with GAL. Identification of these cellular targets and clarifying their contributions to the host-pathogen interaction may be useful for the development of novel therapeutic and prevention strategies against CSBV infection.

Keywords: CSBV; Capsid protein VP3; Galectin; Protein interaction; Yeast two-hybrid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bees
  • Capsid Proteins / metabolism*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line
  • Gene Library
  • Host-Pathogen Interactions
  • Humans
  • Immunoprecipitation
  • Larva
  • Protein Binding
  • Protein Interaction Mapping* / methods
  • RNA Virus Infections / metabolism*
  • RNA Virus Infections / virology*
  • RNA Viruses / metabolism*
  • Two-Hybrid System Techniques*

Substances

  • Capsid Proteins
  • Carrier Proteins

Supplementary concepts

  • Sacbrood virus