Background: Despite of intensive efforts, the tumor progression of ovarian cancer has not yet been fully understood. Various studies suggest that an effective experimental model system In Vitro to mimic microenvironment in vivo and the fabrication of cell pattern formation In Vitro are of great importance for the ovarian cancer research.
Methods: This study cultivates SKOV3, A2780 and A2780/DDP ovarian cancer cell lines in RADA16-I peptide hydrogel to obtain 3D-precultured cancer cells and fabricate a type of miniature cell pattern formation In Vitro on RADA16-I peptide nanofibercoated coverslip by in situ AFM analysis and chemosensitivity assay in vitro.
Results: SKOV3, A2780 and A2780/DDP cells were regularly cultivated in RADA16-I peptide hydrogel, respectively, and present defined cellular characteristics, such as small cell colonies, cell clusters and cell aggregates, etc. On coverslip coated with RADA16-I peptide nanofiber, three 3D-precultured cell lines were flat and spread on coverslips and the regular topographic images by AFM were obtained. The chemosensitivity assay of three ovarian cancer cell lines were significantly different in RADA16-I peptide-coated coverslips compared with 2D dish plates.
Conclusions: By 3D cell culture of ovarian cancer cell lines in RADA16-I peptide hydrogel In Vitro, the cell culture model systems was fabricated. The miniature cell pattern formation In Vitro on peptide nanofiber-coated coverslip was particularly useful experiemental model systems to be served as a valuable methodology for studying ovarian cancer biology.