Proteomics analysis identified peroxiredoxin 2 involved in early-phase left ventricular impairment in hamsters with cardiomyopathy

PLoS One. 2018 Feb 13;13(2):e0192624. doi: 10.1371/journal.pone.0192624. eCollection 2018.

Abstract

Given the hypothesis that inflammation plays a critical role in the progression of cardiovascular diseases, the aim of the present study was to identify new diagnostic and prognostic biomarkers of myocardial proteins involved in early-phase cardiac impairment, using proteomics analysis. Using the two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) combined with MALDI-TOF/TOF tandem mass spectrometry, we compared differences in the expression of proteins in the whole left ventricles between control hamsters, dilated cardiomyopathic hamsters (TO-2), and hypertrophy cardiomyopathic hamsters (Bio14.6) at 6 weeks of age (n = 6, each group). Proteomic analysis identified 10 protein spots with significant alterations, with 7 up-regulated and 3 down-regulated proteins in the left ventricles of both TO-2 and Bio 14.6 hamsters, compared with control hamsters. Of the total alterations, peroxiredoxin 2 (PRDX2) showed significant upregulation in the left ventricles of TO-2 and Bio 14.6 hamsters. Our data suggest that PRDX2, a redox regulating molecule, is involved in early-phase left ventricular impairment in hamsters with cardiomyopathy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight
  • Cardiomyopathies / metabolism*
  • Cardiomyopathies / physiopathology
  • Cricetinae
  • Electrophoresis, Gel, Two-Dimensional
  • Heart Ventricles / metabolism*
  • Heart Ventricles / physiopathology
  • Male
  • Mesocricetus
  • Peroxiredoxins / metabolism*
  • Proteomics*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Peroxiredoxins

Grants and funding

This work was supported by grants from the Grants-in aid for Scientific Research (#26293149 and #15K15236) for SI, (#17H06396) for GI, and (#26293148) for SO. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. There was no additional external funding received for this study.