Influence of reflux ratio on two-stage anoxic/oxic with MBR for leachate treatment: Performance and microbial community structure

Jianbo Liu; Zhiyong Tian; Panyue Zhang; Guanglei Qiu; Yan Wu; Haibo Zhang; Rui Xu; Wei Fang; Jie Ye; Yonghui Song; Guangming Zeng

doi:10.1016/j.biortech.2018.01.146

Influence of reflux ratio on two-stage anoxic/oxic with MBR for leachate treatment: Performance and microbial community structure

Bioresour Technol. 2018 May:256:69-76. doi: 10.1016/j.biortech.2018.01.146. Epub 2018 Feb 2.

Authors

Jianbo Liu¹, Zhiyong Tian², Panyue Zhang³, Guanglei Qiu⁴, Yan Wu⁵, Haibo Zhang¹, Rui Xu¹, Wei Fang¹, Jie Ye¹, Yonghui Song², Guangming Zeng¹

Affiliations

¹ College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
² Department of Urban Water Environmental Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
³ College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; School of Environmental and Chemical Engineering, Chongqing Three Gorges University, Chongqing 404632, China. Electronic address: zhangpanyue@hnu.edu.cn.
⁴ Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117585, Singapore.
⁵ School of Environmental and Chemical Engineering, Chongqing Three Gorges University, Chongqing 404632, China.

PMID: 29428616
DOI: 10.1016/j.biortech.2018.01.146

Abstract

A lab-scale two-stage Anoxic/Oxic with MBR (AO/AO-MBR) system was operated for 81 days for leachate treatment with different reflux ratio (R). The best system performances were observed with a R value of 150%, and the average removal efficiencies of chemical oxygen demand, ammonia and total nitrogen were 85.6%, 99.1%, and 77.6%, respectively. The microbial community were monitored and evaluated using high-throughput sequencing. Proteobacteria were dominant in all process. Phylogenetic trees were described at species level, genus Thiopseudomonas, Amaricoccus, Nitrosomonas and Nitrobacter played significant roles in nitrogen removal. Co-occurrence analyzing top 20 genera showed that Nitrosomonas-Nitrobacter presented perfect positive relationship, as well as Paracoccus-Brevundimonas and Pusillimonas-Halobacteriovorax.

Keywords: Co-occurrence; High-throughput sequencing; Landfill leachate; Microbial community; Phylogenetic tree.

MeSH terms

Biological Oxygen Demand Analysis
Bioreactors
Nitrogen
Nitrosomonas
Phylogeny
Water Pollutants, Chemical*

Substances

Water Pollutants, Chemical
Nitrogen