Intracellular formyl peptide receptor regulates naïve CD4 T cell migration

Biochem Biophys Res Commun. 2018 Feb 26;497(1):226-232. doi: 10.1016/j.bbrc.2018.02.060. Epub 2018 Feb 8.

Abstract

We found that formyl peptide receptor (FPR) 1 and FPR3 were expressed intracellularly and/or the nucleus of naïve CD4 T cell. Activation of naïve CD4 T cells with synthetic intracellular agonists dTAT-WKYMVm and CTP-WKYMVm for FPR members stimulated CD4 T cell migration via pertussis toxin-sensitive manner. Knockdown of FPR1, but not knockdown of FPR3, blocked dTAT-WKYMVm-induced naïve CD4 T cell migration. Stimulation of naïve CD4 T cells with dTAT-WKYMVm elicited the activation of ERK, p38 MAPK, and Akt. Activation of CD4 T cells with anti-CD3 and anti-CD28 antibodies caused surface expression of FPR1 and FPR3, but not FPR2. CD4 T cells isolated from sepsis patients expressed the three members of FPR family on their cell surface. Taken together, our results suggest that intracellular FPR in naïve CD4 T cells and surface FPRs in activated CD4 T cells might regulate immune responses by regulating CD4 T cell activity.

Keywords: CD4 T cells; Formyl peptide receptor; Intraceullar G-protein coupled receptor; Migration; Sepsis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD4-Positive T-Lymphocytes / cytology*
  • CD4-Positive T-Lymphocytes / physiology*
  • Cell Movement / physiology*
  • Cells, Cultured
  • Humans
  • Receptors, Formyl Peptide / metabolism*

Substances

  • FPR1 protein, human
  • FPR3 protein, human
  • Receptors, Formyl Peptide