Background: The present work investigated changes in corn stover pretreated with different white rot fungi. Corn stover was inoculated with Irpex lacteus, Pleurotus ostreatus and Pleurotus cystidiosus prior to incubation under solid-state fermentation conditions at 28 °C for 42 days. Changes in the chemical composition, in vitro rumen degradability, lignocellulolytic enzyme activity and multi-scale structure of the corn stover were analysed.
Results: Content of all lignocellulose components decreased to a certain extent after fungal pretreatment. The total gas production of sterilized corn stover treated with I. lacteus for 42 days increased from 200 to 289 mL g-1 organic matter. Moreover, the cellulase activity was highest at the later stage of I. lacteus pretreatment. Multi-scale structural analysis indicated that white rot fungal pretreatment, and in particular that of I. lacteus, increased and enlarged substrate porosity and caused changes in the structure of corn stover.
Conclusion: Irpex lacteus pretreatment improved the nutritional value of corn stover as a ruminant feed by degrading both cellulose and acid-insoluble lignin as well as changing the structure of the cell walls. © 2018 Society of Chemical Industry.
Keywords: corn stover; in vitro gas production; lignocellulolytic enzymes; structural analysis; white rot fungi.
© 2018 Society of Chemical Industry.