Liver-specific deletion of TSHR inhibits hepatic lipid accumulation in mice

Lingyan Zhou; Kunpeng Wu; Liya Zhang; Ling Gao; Shihong Chen

doi:10.1016/j.bbrc.2018.01.187

Liver-specific deletion of TSHR inhibits hepatic lipid accumulation in mice

Biochem Biophys Res Commun. 2018 Feb 26;497(1):39-45. doi: 10.1016/j.bbrc.2018.01.187. Epub 2018 Feb 6.

Authors

Lingyan Zhou¹, Kunpeng Wu², Liya Zhang³, Ling Gao³, Shihong Chen⁴

Affiliations

¹ Department of Endocrinology, Shandong Provincial Hospital affiliated to Shandong University, Shandong University, Jinan, Shandong, 250021, China; Shandong Provincial Key Laboratory of Endocrinology and Lipid Metabolism, Jinan, Shandong, 250021, China; Institute of Endocrinology and Metabolism, Shandong Academy of Clinical Medicine, Jinan, Shandong, 250021, China; Department of Endocrinology and Metabolism, The Second Hospital of Shandong University, Jinan, Shandong, 250033, China.
² Department of Endocrinology, Shandong Provincial Hospital affiliated to Shandong University, Shandong University, Jinan, Shandong, 250021, China; Shandong Provincial Key Laboratory of Endocrinology and Lipid Metabolism, Jinan, Shandong, 250021, China; Institute of Endocrinology and Metabolism, Shandong Academy of Clinical Medicine, Jinan, Shandong, 250021, China; Department of Hematology, Huashan Hospital, Fudan University, Shanghai, 200040, China.
³ Department of Endocrinology, Shandong Provincial Hospital affiliated to Shandong University, Shandong University, Jinan, Shandong, 250021, China; Shandong Provincial Key Laboratory of Endocrinology and Lipid Metabolism, Jinan, Shandong, 250021, China; Institute of Endocrinology and Metabolism, Shandong Academy of Clinical Medicine, Jinan, Shandong, 250021, China.
⁴ Department of Endocrinology and Metabolism, The Second Hospital of Shandong University, Jinan, Shandong, 250033, China. Electronic address: chenshihong26@163.com.

PMID: 29421660
DOI: 10.1016/j.bbrc.2018.01.187

Abstract

The effect of thyroid-stimulating hormone receptor (TSHR) on hepatic lipid accumulation in vivo is not fully understood. Further, while TSHR in the thyroid has been studied extensively, whether and how the absence of TSHR in the liver affects systemic energy metabolism has not yet been reported. To examine these effects, we generated hepatic TSHR conditional knockout (LT-KO) mice using Cre/LoxP recombination technology. The liver-specific TSHR-knockout (LT-KO) mice exhibited not only lower hepatic triglyceride and cholesterol contents due to modified synthesis and catabolism of lipids in the liver, but also decreased serum lipids, especially serum LDL-C levels. Abnormalities of TSHR in the thyroid affect whole-body energy balance; however, measurements taken in metabolic chambers showed that the hepatic TSHR conditional deletion had no impact on systemic energy metabolism. Unlike its critical role in maintaining the normal growth and function of the thyroid gland, our results demonstrated that hepatic TSHR is involved in liver lipid metabolism and has little effect on energy metabolism.

Keywords: Energy metabolism; Hepatic lipid accumulation; TSHR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Energy Metabolism / physiology*
Lipid Metabolism / physiology*
Liver / metabolism*
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Receptors, Thyrotropin / genetics
Receptors, Thyrotropin / metabolism*
Thyroid Gland / metabolism*

Substances

Receptors, Thyrotropin