Investigation of hydride generation from arsenosugars - Is it feasible for speciation analysis?

Karel Marschner; Stanislav Musil; Ivan Mikšík; Jiří Dědina

doi:10.1016/j.aca.2018.01.009

Investigation of hydride generation from arsenosugars - Is it feasible for speciation analysis?

Anal Chim Acta. 2018 May 30:1008:8-17. doi: 10.1016/j.aca.2018.01.009. Epub 2018 Jan 19.

Authors

Karel Marschner¹, Stanislav Musil², Ivan Mikšík³, Jiří Dědina²

Affiliations

¹ Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 97, 602 00 Brno, Czech Republic; Charles University, Faculty of Science, Department of Analytical Chemistry, Albertov 8, 128 43 Prague, Czech Republic. Electronic address: karel.marschner@biomed.cas.cz.
² Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 97, 602 00 Brno, Czech Republic.
³ Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic.

PMID: 29420947
DOI: 10.1016/j.aca.2018.01.009

Abstract

Hydride generation (HG) from arsenosugars (dimethylarsinoylribosides) in batch and flow injection modes was studied. Its efficiency was found higher in H₂SO₄ medium than in HCl and higher in the batch mode than in the flow injection mode. To increase the efficiency in the flow injection mode a new generator with two inlets of NaBH₄ solution was designed. This modified generator was interfaced between a HPLC column and an atomic fluorescence detector. The arsenosugars studied yielded HG efficiencies in the range 13-30% most probably due to a complicated mechanism of HG. While the mechanism included a formation of two structures of the analyte-borane-complexes, only one of them can lead to a formation of volatile arsanes (dimethylarsane, methylarsane, and arsane were identified).

Keywords: Arsenic; Arsenosugars; High performance liquid chromatography; Hydride generation.