Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene

Stem Cell Res. 2018 Apr:28:44-47. doi: 10.1016/j.scr.2018.01.022. Epub 2018 Feb 2.

Abstract

The leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription factors. The generated iPSC line exhibits expression of common pluripotency markers, differentiates into the three germ layers and has a normal karyotype. The iPSC line can be used to explore the association between LRRK2 mutation and PD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Animals
  • Cell Culture Techniques / methods*
  • Cell Line
  • Female
  • Humans
  • Induced Pluripotent Stem Cells
  • Kruppel-Like Factor 4
  • Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 / genetics*
  • Mice
  • Mutation / genetics*
  • Parkinson Disease / genetics*
  • Parkinson Disease / pathology*

Substances

  • KLF4 protein, human
  • Klf4 protein, mouse
  • Kruppel-Like Factor 4
  • LRRK2 protein, human
  • Leucine-Rich Repeat Serine-Threonine Protein Kinase-2